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The standard concentrations are as follows: 200 、 100 、 50 、 25 、 12
25uL of Matrigel per well was spread in A 24-well cell culture plate
It may contribute to regulating the molecular mechanisms underlying keratinocyte maturation and specialized functions
and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against mitochondrial uncoupling protein 3 (UCP3)
Rat LC3 ELISA Kit Size:96T The standard concentrations are asProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
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